Fig. 5.

Marker expression by neurosphere or differentiated cells with 10 days of culture. a Primary cultured neurospheres in differentiation medium showed positive staining of nestin (green) and Tuj1 (red). b The differentiated cells derived from primary cultured neurospheres showed negative staining of nestin (green) and positive staining of Tuj1 (red). c The differentiated cells derived from primary cultured neurospheres showed positive staining of Tuj1 (red) and glial acidic fibriallary protein (GFAP) (green)