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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Mech Dev. 2010 Dec 1;128(3-4):234–245. doi: 10.1016/j.mod.2010.11.007

Figure 6.

Figure 6

TG2 modulates chondrogenesis through inhibition of the PKA pathway. A - PKA activity is reduced in total cell lysates of 6-day old micromass cultures infected with RCAS-TG2 as compared to control RCAS-GFP micromasses. B- extracellular TG2 decreases cellular levels of cAMP in differentiating chondrocytes, when either overexpressed by RCAS-TG2 virus (black bar) or added in a purified form (0.01U/ml) (open bar); C- Effect of pharmacological kinase inhibitors on deposition of the Alcian Blue-positive cartilaginous matrix in the 5 day old micromass cultures. PKA inhibitors 1 μM KT5720 and 100 nM H89,and PKC inhibitor 10 nM bisindolylmaleimide I (BIM), were continuously present in the medium from day 2 in culture. Cultures treated with DMSO vehicle served as the reference. D - Treatment of micromass cultures with PKA inhibitor H89 reduces transcript levels of xylosyltransferase 2, as measured by real-time PCR. Control cultures were treated with the vehicle (DMSO). (** p<0.01)