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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Mech Dev. 2010 Dec 1;128(3-4):234–245. doi: 10.1016/j.mod.2010.11.007

Figure 7. Regulation of MAPK signaling in differentiating micromass cultures by TG2.

Figure 7

A – activity of ERK and JNK pathways were analyzed with specific luciferase reporter constructs (SABiosciences). Five days-old RCAS-infected micromass cultures were transiently transfected with reporter constructs and luciferase activity was measured 72 hours later using luciferase assay kit (Promega). B – Western blot analysis of phosphorylated p38 detected with specific antibody 9212 (Cell Signaling Technologies, MA). C – treatment of micromass cultures with p38 inhibitor SB203580 (10 μM) reduces transcript levels for xyloxyltransferase 2, measured by real-time RT-PCR (**, p<0.01).