MPP+ rapidly depolarizes DA mitochondria. A, Mitochondria in axons from DA/GFP cultures were labeled with 25 nm TMRE and then assessed before and 30 min after MPP+ treatment. Scale bar: 5 μm B, MPP+ led to significant differences in ΔΨm as measured in arbitrary units (AU; arrows). C, Cross sectional areas of mitochondria labeled with MTR were measured before and after toxin treatment using ImageJ particle analysis. Hatching indicates toxin treatment. B, C, Mean ± SEM of representative determinations from three independent experiments, **p < 0.001.