Table 2.
DA axons exhibit unique mitochondrial and vesicular characteristics
| DA | Non-DA | |
|---|---|---|
| Density‡ | 8.89 ± 0.86 | 9.50 ± 0.82 |
| Moving particles‡ | 1.93 ± 0.22 | 2.49 ± 0.24* |
| Speed (μm/s) | ||
| MTR | 0.28 ± 0.05 | 0.79 ± 0.07** |
| mtDendra2 | 0.30 ± 0.02 | 0.61 ± 0.11** |
| Cross area (μm2) | ||
| MTR | 1.07 ± 0.12 | 2.52 ± 0.11** |
| mtDendra2 | 0.94 ± 0.06 | 2.17 ± 0.47* |
| Ψm (AU) | 58.5 ± 3.22 | 65.2 ± 4.08 |
| Synaptic vesicles | ||
| Moving particles‡ | 3.93 ± 0.30 | 5.71 ± 0.63* |
| Speed (μm/s) | 0.56 ± 0.05 | 0.52 ± 0.03 |
| All particles | ||
| Moving particles‡ | 6.35 ± 1.22 | 6.79 ± 0.48 |
| Speed (μm/s) | 0.12 ± 0.01 | 0.14 ± 0.01 |
‡Number/100 μm. Numbers are mean ± SEM,
*p < 0.05,
**p < 0.001, compared to DA neurons. For MTR studies, 33 dishes from 11 independent experiments were evaluated; for mtDendra2 studies, eight dishes from four independent experiments were assessed. For synaptic vesicles, three dishes from three independent experiments were assessed. When measuring all particles, seven dishes from seven independent experiments were measured. In all cases 3–5 axons per dish were analyzed.