Figure 6.
Restoration of Fmrp in Nestin-expressing aNSCs and their progenies rescues hippocampus-dependent learning deficits. (a, b) Immunohistological analyses of brain sections from cON:Cre:YFP mice at 56 d post-Tam. (a) Red, Fmrp; green, YFP; white, GFAP; blue, DAPI. (b) Red, Fmrp; green, YFP; white, NeuN; blue, DAPI. Left scale bar = 20 μm; Right scale bar = 10 μm. (c, d) Context (c) and Tone (d) trace learning analyses of Cre:YFP Control mice (express Fmrp), cON:Cre:YFP mice (Fmrp restored), and cON:YFP Control (no Fmrp) littermates as determined by the percentage time of freezing to either training context (c) or training tone (d). (e) DNMP-RAM analyses of Cre:YFP Control (has Fmrp), cON:Cre:YFP mice (Fmrp restored), and cON:YFP Control (no Fmrp) littermates as determined by the percentage of correct entry in both separation 2 test and separation 4 test.