Figure 5. In Neurons Eps8 prevalently acts as a capper.

a. Change in RFI and FII (i.e., Eps8:IRSp53:Fa and VASP:IRSp53:Fa normalized with respect to their concentrations in wild type cells) in the various genetic backgrounds. Empty rectangles represent experimental results (see Table 3 in Text S1), filled rectangles reproduce simulations of equations in Table 1 in Text S1 and parameters in Table 2 and Table 3 in Text S1. b. Complexes formed in HeLa cells by Abi1, Eps8, IRSp53, and VASP in the different genetic backgrounds plotted as percentage of total protein concentration in the wild type. Simulations performed as in a. c. Removal of Eps8 from neurons significantly increases the amount of VASP bound to IRSp53. Cortex and hippocampus lysates (1 mg) derived from Eps8 WT or KO mice were immunoprecipitated with anti-IRSp53 or anti Flag as control. Lysates (20 µg) and immunoprecipitates (IPs) were immunoblotted with the indicated abs.