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. 2011 Jul 21;6(7):e21307. doi: 10.1371/journal.pone.0021307

Figure 2. The regulation effect of let-7 target sites on upstream gene, analyzed by dual-luciferase assay system.

Figure 2

The relative Renilla/Firefly ratio of cells transfected with the control plasmid, psiCHECK2 was set to 100%. The Renilla/Firefly values of cells transfected with psiCHECK2-Let7T or psiCHECK2-Let7MT was normalized to its corresponding value of cells transfected with psiCHECK2 in a same independent experiment, respectively. Duplicated experiments were conducted from the procedure of cell culture. Error bars correspond to mean ± SD. n = 3. (** P<0.01).