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. 2011 Jul 21;6(7):e21307. doi: 10.1371/journal.pone.0021307

Figure 3. The capacity of let-7 target sites to regulate E1A expression of the recombinant adenovirus, SG7011let7T.

Figure 3

(A) Virus construction. Eight copies of let-7 target sites were inserted into the 3′UTR of E1A gene and the Ad5 fiber was replaced by Ad5/11 chimeric fiber. ITR, inverted terminal repeats. (B) Detection of E1A protein expression by Western Blotting. The 1, 2, 3 lane represented cells infected with WAd5, SG7011let7T and SG7011let7MT, respectively. PLC, PLC/PRF/5. (C) Detection of E1A mRNA expression by quantitative RT-PCR. The gene of GAPDH was used as an endogenous control and HEK293 cell uninfected with adenovirus was used as a reference sample. Let7T, SG7011let7T; Let7MT, SG7011let7MT. Error bars correspond to mean ± SD. n = 3. (** P<0.01).