Skip to main content
. 2011 Jul 21;7(7):e1002179. doi: 10.1371/journal.pgen.1002179

Figure 4. DAF-12 regulates heterochronic and miRISC gene expression.

Figure 4

(A) Binding sites and ChIP enrichment for DAF-12 relative to ATG within heterochronic and miRISC genes. (B) Promoter regions of corresponding genes were subcloned into pGL3-Luc and luciferase activity was measured in HEK cells upon stimulation for 16 hr with 0.5 µM (25R)-Δ7–DA or DMSO as a control. (C) mRNA levels from synchronized daf-12(rh61rh411) or N2 worms were determined by RT-qPCR. Fold difference is shown relative to N2 mRNA levels (dotted line). (D) mRNA levels from synchronized daf-12(rh284) or N2 worms at L3 stage were determined by NanoString analysis for several heterochronic genes (white bars) or miRISC genes (grey bars). Fold difference is relative to N2 mRNA levels (dotted line). Bars denote standard error of mean (SEM). P-values were determined by Student's t-test. * p<0.05, ** p<0.01, *** p<0.001.