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. 2011 Jul 21;7(7):e1002165. doi: 10.1371/journal.pgen.1002165

Figure 1. Chromatin conformation at the 8q24 locus.

Figure 1

(A) DNase-seq track for the breast cancer cell line MCF-7, the prostate cancer cell line PC3 and the normal-like prostate cell line RWPE-1 displayed on the UCSC genome browser for a 1.7 MB region (chr8: 127,550,000-129,250,000) surrounding the 8q24 cancer susceptibility region, where peaks indicate regions of increased sensitivity to digestions with DNase I. Reads were adjusted for the total number of reads in each experiment. DHSs and promoters used in subsequent analysis are marked by arrows. RefSeq genes are shown, with L being LOC727677. The prostate and breast cancer susceptibility region is depicted by a horizontal red bar. Dotted lines show interactions tested in the chromatin conformation capture experiment shown in (B). (B) Chromatin conformation capture (3C) experiment using the S-DHS as bait in the HB2 breast and the RWPE-1 prostate cell lines. Target sequences are indicated above the lanes. A target sequence just upstream of the EcoRI site 5′ to the bait EcoRI site was used as ligation control (LC). Negative controls for each ligation reaction were cut but unligated DNA fragments. The numbers below the panel refer to interactions shown in (A).