Table 2. Immunohistochemistry, relative quantitation of mRNA MYC expression and MYC gene copy number variation by Taqman and fluorescence in situ hybridization in biopsies of MNU-treated animals.

Treatment	IHQ	mRNA expression(median ± interquartile range)	CNV(number [median ± interquartile range])	Nuclei exhibiting MYC signals (median ± interquartile range)
				1 signal	2 signals	3 signals	4 signals	≥5 signals	HA
Baseline	Negative	-	2 (2.04±0.32)	3±1.5	196±3	1±1	-	-	-
MNU/90th day	Negative	1.52±1.06	2 (1.87±0.55)	3±1.5	194±3	2.5±1.75	-	-	-
MNU/120th daya	Negative	2.24±1.73	2 (1.92±0.74)	4±0.5	188±5	5±4	2±2	-	-
MNU/300th dayb	Positive	3.35±0.09	3 (2.85±0.11)	3.5±0.5	115.5±0.5	59.5±1.5	19±3	2.5±2.5	-
MNU/940th dayc	Positive	4.75	3 (3.12)	3	105	61	16	5	10
Canova/960th dayc	Positive	5.04	3 (3.04)	4	109	52	18	9	8

IHC: immunohistochemistry; CNV: copy number variation; HA: high amplification.

^aFive animals;

^bTwo animals;

^cOne animal.