Table 1. Ratio of oriCII/oriCI in V. cholerae strains.
Media | Strain | Genetype | oriCII / oriCI |
LB* | N16961 | wild type | 1# |
YBB995 | ΔrctA | 1.53±0.06 | |
YBB999 | parS2-B::parS2X | 0.85±0.04 | |
M9† | YBB703 | wild type/vector | 1# |
YBB682 | wild type/rctB+ | 2.48±0.24 | |
YBB2003 | ΔrctA/vector | 1.31±0.06 | |
YBB2004 | ΔrctA/rctB+ | 3.14±0.46 | |
YBB2005 | parS2-B::parS2X/vector | 0.94±0.13 | |
YBB2006 | parS2-B::parS2X/rctB+ | 1.70±0.17 |
*Samples were obtained from mid-log phase cultures at an OD600 of ∼0.7.
†Samples were obtained from cultures grown in M9-Glucose supplemented with 100 µM IPTG for 2.5 hrs.
#Defined as 1.