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. 2011 Jul 21;6(7):e22134. doi: 10.1371/journal.pone.0022134

Figure 3. Effect of temperature on the enzymatic activity of mutants C457D, C457D/A525F, C457D/Y239F and C457D/N211K.

Figure 3

A) Percentage of labeled product per mg of protein produced by homogenates of mutant C457D (♦,⋄), C457D/A525F (▪,□) and C457D/Y239F (▴,▵) at 35°C (filled symbols) and 25°C (empty symbols), respectively. B) Percentage of labeled product per mg of protein produced by homogenates of mutant C457D (♦,⋄) and C457D/N211K (•, ○) at 35°C (filled symbols) and 25°C (empty labels). Values are the means of at list two separate experiments, each one carried out in duplicate.