Table 1. OSC specific activity of the homogenates of the differently transformed SMY8 strains.
SceOSC mutant | * Specific Activity (nmol/h/mg protein) |
C457D | 1.91±0.095 |
C457D/A525F | 0.14±0.036 |
C457D/A525Y | 0.29±0.090 |
C457D/A525I | 0.73±0.140 |
C457D/A525L | 0.75±0.110 |
C457D/H193A | 0.64±0.058 |
C457D/H193N | 0.49±0.077 |
C457D/N211A | 0.65±0.130 |
C457D/N211D | 0.79±0.150 |
C457D/N211Q | 1.00±0.180 |
C457D/N211K | 0.39±0.073 |
C457D/H291A | 0.64±0.140 |
C457D/H291N | 0.80±0.260 |
C457D/T235A | 0.67±0.130 |
C457D/T235C | 0.85±0.130 |
C457D/Y239A | 0.97±0.130 |
C457D/Y239F | 0.34±0.044 |
*Values ± SE are the means of duplicate assays of at least two different experiments.