Table 1. Steady state characteristics of wild type and ▵icl strains of M.bovis BCG (BCG) and wild type M. tuberculosis (MTB) grown in a glycerol-limited chemostat.
Organism | BCG | MTB | ||
Strain | Wild type | ▵icl | Wild type | |
Dilution rate (h−1) | 0.01 | 0.03 | 0.03 | 0.01 |
Biomass (g dry weight l−1) | 1.40 | 0.70 | 0.30 | 1.28 |
CFU (×106 ml−1) | 164 | 51.0 | 26.85 | 144 |
Specific glycerol consumption rate (mmol g biomass−1 h−1) | 0.39 | 0.74 | 0.57 | 0.39 |
Specific Tween 80 consumption rate (mmol g biomass−1 h−1) | 0.002 | 0.09 | 0.07 | 0.003 |
Specific CO2 production rate (mmol g biomass−1 h−1) | 0.57 | 0.45 | 0.69 | 0.23 |
Protein content (g l−1) | 22.91* | 21.40* | 32.74 | - |
Carbohydrate content (g l−1) | 29.20* | 21.91* | 14.37 | - |
Lipid content (g l−1) | 33.90* | 44.82* | 48.14 | - |
Yield (g bacteria g glycerol−1) | 0.30 | 0.46 | 0.56 | 0.22 |
Values are the averages from duplicate chemostat cultures and three independent measurements. * Data from Beste et al. (2005).