Figure 7. Loss of ethanol fermentation does not affect survival of A. fumigatus infected mice.

(A) (B) Outbred CD-1 mice were immunosuppressed by intraperitoneal (i.p.) injection of 175 mg/kg cyclophosphamide 2 days prior to infection and 150 mg/kg 3 days post-inoculation, and subcutaneous (s.c.) injection of Triamcinolone (40 mg/kg) 1 day prior to infection and 6 days post-infection. Mice were inoculated with ∼10⁴ conidia of the indicated strains by inhalation of an aerosol in a Hinner's chamber. (C) For the triamcinolone model outbred CD-1 mice were immunosuppressed on day -1 by s.c. injection of Kenalog (40 mg/kg), followed by inhalation-inoculation with 10⁴ conidia on day 0. (D) gp91^phox−/− mice were challenged intranasally with 10⁶ conidia in a volume of 25 µl of the indicated strains. The ethanol deficient mutant strains, ΔalcC and ΔpdcA, showed no difference in mortality compared to the wild type or reconstituted strains in any of the tested IPA models (p>0.2, Log-Rank Test). 10 animals were inoculated per experiment per inoculation group and the experiments were repeated in duplicate.