Figure 1. Vhl and Hif1α gene expression in tamoxifen-fed Vhl^floxed-UBC-Cre-ER^T2 and Hif1α^floxed-UBC-Cre-ER^T2 mice.

(A) Vhl^wt-UBC-Cre-ER^T2 (n = 3), Vhl^floxed (n = 6) and Vhl^floxed-UBC-Cre-ER^T2 (n = 6) mice were placed on a tamoxifen diet for ten days followed by ten additional days on a normal diet. Gene expression was assessed by RT-PCR in the tissues indicated, the expression of the Vhl gene was normalized to that of Hprt and it was expressed as the change relative to Vhl^floxed mice. (B) Tamoxifen intake was measured over the 10 days of tamoxifen administration in Vhl^wt-UBC-Cre-ER^T2 (n = 3), Vhl^floxed (n = 6) and Vhl^floxed-UBC-Cre-ER^T2 (n = 6) mice. (C,E) Hif1α^wt-UBC-Cre-ER^T2 (n = 4), Hif1α^floxed (n = 3) and Hif1α^floxed-UBC-Cre-ER^T2 (n = 5) mice were administered tamoxifen as indicated above. Hif1α (C) or Vhl (E) gene expression was normalized to that of Hprt and expressed as the change relative to Hif1α^floxed mice. (D) Tamoxifen intake in Hif1α^wt-UBC-Cre-ER^T2 (n = 4), Hif1α^floxed (n = 3) and Hif1α^floxed-UBC-Cre-ER^T2 (n = 5) mice was measured as in B. Total intake per day was expressed relative to the body weight at the end of the tamoxifen treatment and the values represent the mean ± SEM. Statistical significance was assessed using a two-tailed Student's t-test, (*, p<0.05; **, p<0.01) when comparing Vhl^wt-UBC-Cre-ER^T2 or Hif1α^wt-UBC-Cre-ER^T2 with Vhl^floxed-UBC-Cre-ER^T2 or Hif1α^floxed-UBC-Cre-ER^T2 respectively; (^##, p<0.01) when comparing Vhl^floxed or Hif1α^floxed with Vhl^floxed-UBC-Cre-ER^T2 or Hif1α^floxed-UBC-Cre-ER^T2 respectively.