Table 1. Combination of primary and secondary antibodies for double and triple-fluorescent staining procedures.

Series	Primary antibodies			Secondary antibodies
	Antigen	Host	Dilution	Donkey IgG	Dilution
ChAT/c-Fos/GluT(EAAC1)	ChAT	goat	1∶200	Anti-goat-FITC	1∶200
	c-Fos	rabbit	1∶10000	Anti-rabbit-CY5	1∶200
	GluT(EAAC1)	mouse	1∶500	Anti-mouse-TRITC	1∶200
c-Fos/GluT(EAAC1)	c-Fos	rabbit	1∶10000	Anti-rabbit-CY5	1∶200
	GluT(EAAC1)	mouse	1∶1000	Anti-mouse-TRITC	1∶200

Abbreviations: ChAT: choline acetyltransferase; GluT(EAAC1): glutamate transporter.