Table 4. Density and diameter of ChAT+ varicosities and length of ChAT+ fiber segments within microproximity of Pyramidal cells in Layer V of mPFC.
| IL | PrL | Cg1 | |
| Visual/HDB stimulation | |||
| Microproximity with c-Fos+ pyramidal cells | |||
| Density of ChAT+ varicosities | 0.51±0.06* & | 0.49±0.06* & | 0.48±0.03* & |
| Diameter of ChAT+ varicosities(nm) | 608±10& | 602±14 | 601±28 |
| ChAT+ fiber length(µm) | 9.9±1.1 | 9.7±1.5 | 9.4±2.2* |
| Microproximity with non-activated pyramidal cells | |||
| Density of ChAT+ varicosities | 0.34±0.02* | 0.34±0.05* | 0.34±0.03* |
| Diameter of ChAT+ varicosities(nm) | 626±36▴ | 598±24 | 608±20 |
| ChAT+ fiber length(µm) | 9.1±0.9▴ | 8.3±0.6▴ | 7.1±0.4▴ |
| Control | |||
| Microproximity with non-activated pyramidal cells | |||
| Density of ChAT+ varicosities | 0.31±0.03& + ‡ | 0.34±0.02& | 0.32±0.04& |
| Diameter of ChAT+ varicosities(nm) | 581±6& ▴ | 580±16 | 596±15 |
| ChAT+ fiber length(µm) | 12.9±1.1▴ + | 10.9±0.9▴ | 10.1±0.7▴ |
All values are means ± SEM in visual/HDB stimulation rats and control rats. Density of ChAT+ varicosities is expressed in number of varicosities/µm of ChAT+ fiber segment. Diameter of ChAT+ varicosities is expressed in nm. ChAT+ fiber length is expressed in µm. Abbreviations: Cg1: cingulate cortex; ChAT: choline acetyltransferase; IL: infralimbic cortex; PrL: prelimbic cortex; GluT(EAAC1):glutamate transporter; HDB: horizontal limb of the diagonal band; mPFC: medial prefrontal cortex.
*p≤0.05, Mann-Whitney U test, two-tailed, comparing microproximity of Pyramidal/c-Fos+ cells to microproximity of Pyramidal cells not immunoreactive to c-Fos within visual/HDB stimulation group.
p≤0.05, Mann-Whitney U test, two-tailed, comparing microproximity of Pyramidal/c-Fos+ cells in visual/HDB stimulation group to microproximity of Pyramidal cells not immunoreactive to c-Fos in control group.
p≤0.05, Mann-Whitney U test, two-tailed, comparing microproximity of Pyramidal cells not immunoreactive to c-Fos in visual/HDB stimulation group to control group.
p≤0.05, Mann-Whitney U test, two-tailed, comparing microproximity of Pyramidal/c-Fos+ cells in visual stimulation group to microproximity of Pyramidal cells not immunoreactive to c-Fos in control group.
p≤0.05, Mann-Whitney U test, two-tailed, comparing microproximity of Pyramidal/c-Fos+ cells in HDB stimulation group to microproximity of Pyramidal cells not immunoreactive to c-Fos in control group.