Table 1. Immunohistochemistry: Antibodies, origins and procedures.
Antibody | Type | Origin | Dilution | Incubation time (mn) | Detection |
CD 163 | monoclonal mouse | NOVOCASTRA Réf-NCL 163 | 1/100 | 60 | ENV MOUSE DAKO K4007 |
NOX2 GP91-phox | monoclonal mouse | Marck Queen USA | 1/300 | 60 | ENV MOUSE DAKO K4007 |
P22-phox | polyclonal rabbit | SANTA CRUZ Ref.sc-20781 | 1/250 | 60 | ENV RABBIT DAKO K4011 |
CX43 | monoclonal rabbit | CELL SIGNALING Ref.3512 | 1/50 | overnight | ENV RABBIT DAKO K4011 |
CD 34 | monoclonal mouse | DAKO Ref.M7165 | 1/100 | 60 | ENV MOUSE DAKO K4007 |
Alpha-SMA | monoclonal mouse | DAKO Ref.M0851 | 1/50 | 30 | ENV MOUSE DAKO K007 |
CD68KP1 | monoclonal mouse | DAKO Ref.N1577 | 1/400 | 60 | ENV MOUSE DAKO K4007 |
CD3 | monoclonal mouse | DAKO Ref.M7254 | 1/50 | 60 | ENV MOUSE DAKO K4007 |
Ki 67 | monoclonal mouse | DAK0 Ref.7240 | 1/40 | 30 | CSA II DAKO 1497 |