Figure 4.
HAND2 protein localization in Day 3.5, 4.5 and 5.5 of pregnant mouse uteri using immunohistochemistry.(A-D) Sections from pre-implantation sites from Day 3.5 pregnant mouse uteri. Sections from non-implantation (NIS) segments (E,F) and implantation (IS) segments (G-I) from Day 4.5 pregnant mouse uteri. Sections from non-implantation (NIS) segments (J,K) and implantation (IS) segments (L-O) from Day 5.5 pregnant mouse uteri. All sections are oriented with mesometrium (m) downward and numbers above scale bars are in microns. Abbreviations: ge, glandular epithelium; b, blastocyst; le, luminal epithelium; pdz, primary decidual zone. These results are representative of at least 3 independent samples. Global linear adjustments of the brightness and color level were made on the photomicrographs to more accurately represent what was seen on the slides under the microscope.