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. Author manuscript; available in PMC: 2012 Jan 10.
Published in final edited form as: Vet Parasitol. 2010 Oct 7;175(1-2):33–39. doi: 10.1016/j.vetpar.2010.09.030

Fig. 1.

Fig. 1

Polymorphisms at 9 genetic markers by direct PCR-DNA sequencing of Toxoplasma gondii isolates from pigs from Brazil

DNA Sequence analysis at A) c22-8, c29-2, L358, PK1; B) SAG1, SAG2, BTUB, B1 and C) GRA6. Consensus sequence is defined as the nucleotide shared by at least two of the three archetypal Type I, II and III strain alleles. Numerical positions refer to sites in the published sequences (GenBank accession no. EU258475, EU258490, L42007, X14080, M33572, AY143127, AF179871 and F239283 for c22-8, L358, PK1, SAG1, SAG2, BTUB, B1 and GRA6 respectively). For c29-2, the amplicon was blasted (using the BlastN algorithm) at ToxoDB (www.toxodb.org) and the corresponding RH sequence used as the reference. “.” indicates identity with consensus; “-” indicates an insertion/deletion “u” indicates a nonarchetypal allele; I, II or III refers to an archetypal allelic sequence from a Type I, II or III strain.