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. Author manuscript; available in PMC: 2011 Aug 15.
Published in final edited form as: Exp Cell Res. 2010 May 24;316(14):2322–2339. doi: 10.1016/j.yexcr.2010.05.020

Figure 8. Dermal fibroblasts lacking sdc1 synthesize increased levels of FN and assemble DOC-insoluble FN fibrils.

Figure 8

A. Wild type and sdc null dermal fibroblasts were immunostained for total FN (green); actin (red) and nuclei (blue) were visualized with rhodamine phalloidin and DAPI, respectively. Both wild type and sdc1 null dermal cells assemble FN fibrils at the cell surface. B. Sdc1 null dermal fibroblasts exhibit both increased DOC-soluble and insoluble FN relative to wild type dermal fibroblasts. Sdc1 null dermal fibroblasts also expressed more total FN than their wild type counterparts. Quantification is expressed relative to actin and to wild type dermal fibroblasts. Shown is a representative blot and quantification of that blot for each experiment. Scale bars, 40 µm and 20 µm (A).