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. 2011 Mar 11;39(13):5449–5458. doi: 10.1093/nar/gkr097

Figure 3.

Figure 3.

Effect of HMGB1 or supercoiled DNA on tetrasome dissociation by Asf1. (A) Effect of HMGB1 on stability of tetrasomes in the presence of yAsf1. HMGB1 was added to give a final concentration of 0, 0.4, 0.8, 2 and 4 µM with 0.4 µM 5SDNA and 601 tetrasomes. The gels were scanned and processed as in Supplementary Figure S1, with fluorescence of H3/H4*FM and SYBR Green I stained DNA shown in green, and yAsf1* in red. (B) DNA competition assay. Supercoiled or linear 5SDNA or 601 plasmid DNA was added at 0.02 µM concentration to tetrasomes in the absence or presence of yAsf1. The images were produced as in (A).