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. 2011 Mar 11;39(13):5449–5458. doi: 10.1093/nar/gkr097

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Affinity of Asf1 and H3/H4 determined by fluorescence quenching. (A) Schematic diagram of model equilibria for yAsf1 association with H3/H4 complexes. D and T indicate H3/H4 dimers and tetramers, respectively. (B) H3/H4*^Qsy9 binding to yAsf1* was observed by fluorescence quenching. H3/H4*^Qsy9 was titrated into 1.0 nM yAsf1*. The data were fitted with a ligand-depleted binding model (Equation 1; GraphPad Prism) because the concentration of yAsf1* was within 10-fold of the K_d value. (C) H3/H4*^Qsy9 binding to yAsf1*^V94R was observed by fluorescence quenching. H3/H4*^Qsy9 was titrated into 1.0 nM yAsf1*^V94R. The curve was fitted (GraphPad Prism) with a Langmuir single-site binding isotherm.