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. 2011 Mar 12;39(13):5538–5554. doi: 10.1093/nar/gkr124

Figure 1.

Figure 1.

Inhibition of L1 retrotransposition by A1 proteins. (A) 293T cells were co-transfected with 0.5 µg of the expression plasmids for APOBEC family proteins and 1.5 µg of L1 retrotransposition indicator construct pL1RP-EGFP. After 24 h, cells were subjected to puromycin (1.0 µg/ml) selection. GFP expression within the transfected 293T cells was analyzed on flow cytometry, after 7–9 days of puromycin selection. Relative retrotransposition frequency in the absence of APOBEC proteins (vector) was set as 1.0. The histogram bars represent the mean of three independent cultures, and the standard deviation is shown. (B) Western blot analysis was performed by using extracts from 293T cells transfected by the expression plasmids for APOBEC family proteins and detected by using antibodies specific for the epitopes present in the test proteins. (C) The transposition frequency of L1 in 293T cells co-transfected with 1.5 µg of pL1RP-EGFP along with the expression plasmids for APOBEC family proteins as described in (A), except for that the amounts of APOBEC-expression plasmids were varied (0.5, 0.25 and 0.125 µg). The histogram bars represent the mean of three independent cultures, and the standard deviation is shown. (D) Western blot analysis of the protein expression levels from the experiment in (C).