Figure 1.
Specificity of JBP1 for J-DNA over T-DNA is salt dependent. (A and B) Binding of varying concentrations of Lt-JBP1 to J-DNA (A) and T-DNA (B) measured by the anisotropy of the signal of 1 nM labeled DNA, for seven different (buffered) concentrations of NaCl; symbols indicate the data points and vertical bars, the measures standard deviation; lines represent the fitted function that determination of the allowed KD; for very tight KD the fit is poorer since it is likely to be far from the assumed equilibrium. (C) The ratio of the calculated KD for T-DNA over J-DNA (indicating the specificity toward J-DNA) is shown as the function of the ionic strength of the buffer; the dotted line represents a polynomial fit to the data without assuming a physical model.