Figure 5.

Effect of Mg²⁺ ions on the interaction of Rpp20, Rpp25 and Pop5 with H1 RNA. (A–C) Gel shift assays were performed as describe in Figures 3 and 4 in the presence or absence of 10 mM MgCl₂ in the binding buffer. Protein concentrations for Rpp20 were 0, 0.12, 0.24, 0.48, 0.96, 1.92 and 3.84 µM, for Rpp25 were 0, 0.03, 0.06, 0.12, 0.24, 0.48 and 0.96 µM and for Pop5 were 0, 0.125, 0.25, 0.5, 1, 2 and 4 µM. (D) Effect of increasing concentrations of MgCl₂ on binding of Rpp20/Rpp25 to H1 RNA. The two proteins were added at equimolar ratio, 0.8 µM each. The ternary complexes were quantitated and plotted.