Figure 1.

(A) shRNAs were designed on human NF-YA and NF-YB genes, targeting exons 6 and 5, respectively (arrows). (B) Transcription (left panel) and protein levels (right panel) of NF-YA and NF-YB subunits after 48 h of shRNA lentiviral infection of HCT116 cells. (C) Left panel: growth rate of control and shRNA-infected HCT116 cells. Forty eight hours post-infection cells were trypsinized and equal number of cells were seeded into new culture dishes (time = 0 h). Viable cells were estimated after 12, 24, 48 and 72 h post-seeding. Right panel: western blot analysis of total extracts of control and shRNA infected cells. Actin was used as loading control.