Figure 4.
Schematic representation of a biochemical pathway under the control of the rpoS gene: arginine degradation to succinate via putrescine (adapted from EcoCyc; http://ecocyc.org/). Genes belonging to this pathway and found to be preferentially transcribed by EσS in ROMA experiments are boxed with solid lines (speB, puuB and gabD); genes co-transcribed with either puuB or gabD (i.e. belonging to the puuCBE and gabDTP operons; see also Supplementary Table 1) are boxed with dashed lines. Other genes of the pathway known to be dependent on the rpoS gene in vivo (puuA and puuD) (20,55) are underlined.