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. 2011 Mar 21;39(13):5513–5525. doi: 10.1093/nar/gkr131

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Overexpression of the novel toxins inhibits translation in E. coli. The DJ624Δara strain containing the pBAD33 (1), pBAD-parE_RK2 (2), pBAD-yoeB (3), pBAD-ecoT1_EDL933 (4), pBAD-mavT1_K10 (5), pBAD-spyT1₁₀₂₇₀ (6), pBAD-spyT2₁₀₂₇₀ (7), pBAD-bceT1_E33L (8), pBAD-smeT1₁₀₂₁ (9), pBAD-spyT1_M1 (10), pBAD-bceT5_E33L (11), pBAD-nspT1_PC7120 (12), pBAD-nspT2_PC7120 (13), pBAD-spyT4₁₀₂₇₀ (14), pBAD-spyT5₁₀₂₇₀ (15), pBAD-spyT3₁₀₂₇₀ (16), pBAD-spyT1₉₄₂₉ (17) and pBAD-lmoT1_EGD-e (18) were grown in M9 medium. After induction of toxin expression by arabinose addition, cultures were labeled with ³⁵S-methionine. Translation rate was measured as described in ‘Materials and Methods’ section.