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. 2011 Mar 16;39(13):5647–5657. doi: 10.1093/nar/gkr145

Figure 4.

Figure 4.

LIN-29/mCherry accumulates precociously in pabp-2(RNAi) animals. Strain HW761 expressing lin-29b/mCherry was exposed to RNAi by feeding as synchronized L1-stage larvae. (A and C–F) Photomicrographs of animals grown on the indicated RNAi. Upper panels show Nomarski micrographs and lower panels show fluorescence micrographs. Arrowheads in the Nomarski micrographs point to the distal tip of the gonad, white arrowheads in the fluorescence micrographs point to seam cell nuclei. (A) Mock-treated L4 animal accumulating LIN-29B at mid L4. The brackets indicate the nuclei of the daughter cells of the final seam cell division. (B) Percentage of animals exhibiting precocious seam cell fusion (white bars) and LIN-29B accumulation in seam cell nuclei at late L3. The number of animals examined is indicated above the bars. (C–F) Late L3 stage animals exposed to (C) mock RNAi, (D) lin-14(RNAi), (E) lin-41(RNAi) and (F) pabp-2(RNAi).