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. 2011 Mar 16;39(13):5555–5567. doi: 10.1093/nar/gkr147

Figure 1.

Figure 1.

Schematic representation of mRNAs containing various 5′-UTR sequences upstream of the luciferase coding region. mL913Fluc includes a 913 nt long 5′-UTR consisting of the full-length leader sequence of the human LINE-1 retrotransposon mRNA. The other 10 mRNAs have various deletions in the 5′-UTR, but all contain the same 45 nt 5′-terminal portion of the LINE-1 ORF fused to the Fluc coding sequence and the same 52 nt 3'-UTR (see Materials and methods). The length of the 5′-UTRs in each mRNA is indicated in nucleotides (nts). See Supplementary Figure S1 for 5′-UTRs secondary structure. mL526EMCVFluc, mL386EMCVFluc, mL200EMCVFluc and mL5EMCVFluc were prepared by the insertion of 488 nt EMCV IRES fragments into 5′-UTRs of mL526Fluc, mL386Fluc, mL200Fluc and mL5Fluc constructs, respectively.