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. 2011 Mar 16;39(13):5555–5567. doi: 10.1093/nar/gkr147

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Determination of full-translation time for mRNAs with different length 5′-UTRs. Time course of luciferase synthesis in the wheat germ system (effective K⁺ concentration is 35 mM) translating the indicated mRNAs. Dashed lines represent the Gaussian fitting of the second derivatives of the corresponding kinetic curves (see legend to Figure 2).