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. 2011 Mar 16;39(13):5555–5567. doi: 10.1093/nar/gkr147

Figure 4.

Figure 4.

Linear dependence of full-translation time on 5′-UTR length. (A) Fluc mRNAs with 5′-UTRs of different lengths were translated in the wheat germ cell-free system at 25°C with effective K+ concentration adjusted to either 35 mM (open symbols) or 75 mM (solid symbols). Error bars denote the sum of the standard errors derived from the curve fitting procedure and the dispersion of data obtained in three independent experiments. The lines represent the least squares approximation of data points. (B) The same mRNAs were translated in the Krebs-2 cell-free system at 30°C (open symbols). Four Fluc mRNAs with 5′-UTRs of different length with the EMCV IRES sequence inserted adjacent to AUG codon were translated in the same system (solid symbols).