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. 2011 Mar 16;39(13):5555–5567. doi: 10.1093/nar/gkr147

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — The effect of K⁺ addition during the translation reaction on full-translation time. (A) The K⁺ concentration in the wheat germ system translating 25 nM mL5Fluc mRNA (5 nt 5′-UTR) was increased from 35 mM to 75 mM at the indicated times after the start of the translation reaction. (B) The same as (A), but in the system translating mL913Fluc mRNA (913 nt 5′-UTR). (C) Dependence of full-translation time on the time of K⁺ addition after the start of the translation reaction. At the indicated time points, the final concentration of K⁺ was increased from 35 mM to 75 mM in the wheat germ system translating 25 nM mL5Fluc mRNA (open symbols) or mL913Fluc mRNA (solid symbols). Dashed lines indicate the translation times measured without extra K⁺ addition.