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. 2011 Mar 22;39(13):5682–5691. doi: 10.1093/nar/gkr155

Figure 2.

Figure 2.

miR-423-5p inhibits PR transcription. (A and B) RT–qPCR showing inhibition of (A) PR mRNA and (B) PR hnRNA expression by miR-423-5p. (C and D) ChIP-qPCR analysis of (C) RNA Pol II and (D) H3K9me2 occupancy on the PR promoter following treatment with miR-423-5p. miRNA mimics were added to cells at 25 nM for RT–qPCR and 50 nM for ChIP-qPCR. Error bars indicate SD (n = 3). P-values were calculated using the two-tailed unpaired Student's t-test with equal variances. **P < 0.01.