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. 2011 Mar 22;39(13):5682–5691. doi: 10.1093/nar/gkr155

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Inhibition of PR by miR-423-5p requires seed sequence complementarity. (A) Alignment of miR-423-5p with ncRNA overlapping the PR promoter (seed sequence shown in lower case). (B) PhastCons conservation analysis of the miR-423-5p target site within the PR promoter (seed sequence target site underlined, sequence listed 3′ to 5′). (C) Alignment of mutant miR-423-5p mimics with ncRNA overlapping the PR promoter (mismatched bases underlined). (D) RT–qPCR measuring PR mRNA expression following treatment with mutant miR-423-5p mimics. (E) RT–qPCR measuring PR mRNA expression following treatment with a DNA analog of miR-423-5p. Duplexes were added to cells at 25 nM. Error bars indicate SD (n = 3). P-values were calculated using the two-tailed unpaired Student's t-test with equal variances. **P < 0.01.