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. 2011 Mar 22;39(13):5682–5691. doi: 10.1093/nar/gkr155

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© The Author(s) 2011. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Inhibition of PR expression by miR-423-5p is mediated by AGO2. (A and B) RIP analysis of (A) AGO1 and (B) AGO2 recruitment to the ncRNA overlapping the PR promoter by miR-423-5p. (C) RT–qPCR analysis of AGO1 mRNA expression following treatment with siRNA against AGO1. (D) RT–qPCR analysis of PR mRNA inhibition by miR-423-5p following AGO1 knockdown. (E) RT–qPCR analysis of AGO2 mRNA expression following treatment with siRNA against AGO2. (F) RT–qPCR analysis of PR mRNA inhibition by miR-423-5p following AGO2 knockdown. TF1 = transfection 1, TF2 = transfection 2. siRNAs and miRNA mimics were added to cells at 25 nM for AGO reversal experiments and 50 nM for RIP. Error bars indicate SD (n = 3). P-values were calculated using the two-tailed unpaired Student's t-test with equal variances. **P < 0.01.