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. 2003 Nov 5;101(1):16–22. doi: 10.1073/pnas.2235688100

Fig. 7.

Fig. 7.

Knockdown of Crx and Nrl expressions in the mouse retina using RNAi vectors. (A) Specific down-regulation of the expression of Crx, Nrl, GAPDH, and rhodopsin in the RNAi vector transfected mouse retinae. CD1 mouse retinae were coelectroporated in vivo with pCAG-GFP (2 μg/μl) and an RNAi vector (U6, U6-Crx, U6-Nrl or U6-GAPDH; 4 μg/μl) at P0, harvested at P10, and dissociated into single cells. The dissociated cells were stained with anti-Crx, anti-Nrl, anti-GAPDH, or anti-rhodopsin antibody, and the numbers of positive cells were scored. Both GFP-positive (green bars) and GFP-negative (yellow bars) cells were analyzed. (B) Morphology of the retinal cells transfected with RNAi vector. CD1 mouse retinae were coelectroporated in vivo with pCAG-GFP and an RNAi vector at P0, harvested at P20, and sectioned. Sections were stained with anti-rhodopsin antibody (red) and 4′,6-diamidino-2-phenylindole (blue), and images were taken with a confocal microscope (Zeiss LSM 510). (Insets) Higher-magnification views of OSs. (Magnifications: ×800.)