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Analysis of reverse differential counting for a passivated capture chamber. (a) Percent error decreases exponentially (dashed line, R2 = 0.57) as the number of leukocytes entering the chip (forward count) increases. (b) Cumulative counts of forward and reverse-flow regimes for the particular experiment highlighted in (a). Error is calculated by normalizing the differential count (absolute difference between the forward and reverse counts) to the forward count.
