Skip to main content
. 2003 Dec 19;101(1):43–47. doi: 10.1073/pnas.0307229101

Fig. 1.

Fig. 1.

Effect of dominant-negative A-C/EBP on the differentiation of 3T3-L1 preadipocytes. Nearly confluent 3T3-L1 preadipocytes were infected with adenoviruses either lacking an insert (control) or containing an insert that encodes A-C/EBP, a dominant-negative C/EBP. Exposure to the viruses was continued for 2 days, at which time (2 days postconfluent) the preadipocytes were induced to differentiate by using the standard protocol. (A) Eight days after induction, the cell monolayers were stained with Oil red O. (B) Four days after induction, cell lysates were immunoblotted with antibody specific against C/EBPα, PPARγ, and 422/aP2.