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Effect of A-C/EBP on the expression of cell-cycle markers. 3T3-L1 preadipocytes were infected with control or A-C/EBP adenoviruses and induced to differentiate as in Fig. 1. Cell lysates were prepared every 4 h after induction differentiation. Cell lysates were subjected to SDS/PAGE and immunoblotted with antibodies against cyclin D1, cyclin B1, p27/Kip1, cyclin A, Cdk2, C/EBPβ, and Flag (recognizing Flag-A-C/EBP fusion protein).
