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. 2003 Dec 15;101(1):82–86. doi: 10.1073/pnas.2237114100

Fig. 1.

Fig. 1.

Characterization of the Drosophila parg mutant. (A) The upper line denotes genes in the parg locus predicted by the European Drosophila Genome Sequencing Consortium (GenBank accession no. Z98254). The structure of the Drosophila parg gene is shown in the lower line, and the parg27.1 mutant deletion is indicated by the bracket. Boxes denote exons, and filled boxes denote coding regions. The position of a P element insertion is shown. (B) Genomic Southern blotting of the parg gene. Lane 1, parg27.1/Y; lane 2, wild type. (C) parg transcripts examined by RT-PCR. Lane 1, parg27.1/Y; lane 2, wild-type. rp49 was used as a control. (D) Effects of temperature on development of parg mutants. (E) Relative content of poly(ADP-ribose) examined by ELISA using an anti-poly(ADP-ribose) antibody.