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. 2003 Dec 22;101(1):99–104. doi: 10.1073/pnas.0307598100

Fig. 1.

Fig. 1.

PIAS3 can activate Smad transcriptional activity. (A) Different PIAS proteins have distinct effects on the SBE4-Luc reporter gene activity. HaCaT cells were cotransfected with the SBE4-Luc reporter gene and a Flag-PIAS plasmid as indicated. Cells were treated with or without TGF-β and analyzed for luciferase activity. (B) The short and long forms of PIAS3 have comparable activity to stimulate the SBE4-Luc reporter gene. (C) PIAS3 can activate the transcriptional activity of GAL4-Smad fusion proteins. HaCaT cells were cotransfected with the GAL4-Luc reporter gene along with the GAL4 DNA-binding domain (DBD), GAL4-Smad2, GAL4-Smad3, or GAL4-Smad4 in the absence or presence of Flag-PIAS3 plasmid, treated with or without TGF-β, and analyzed for luciferase activity. (D) The stimulatory effect of PIAS3 occurs through the transcriptional activation domains of Smad proteins.