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. 2003 Dec 22;101(1):99–104. doi: 10.1073/pnas.0307598100

Fig. 3.

Fig. 3.

The RING domain of PIAS3 is essential for interaction with p300/CBP. (A) PIAS3 can interact with p300/CBP. Myc-PIAS3 along with p300-HA or CBP-HA and TβRI (T204D) for TGF-β stimulation was cotransfected into 293T cells. (B) The RING domain of PIAS3 can interact with p300. (C) The RING domain mutant PIAS3 cannot bind to p300. COS cells were cotransfected with p300-HA along with Flag-tagged wild-type PIAS3 or RING mutant PIAS3 (C299S, H301A) in the absence or presence of TβRI (T204D) for TGF-β stimulation. (D) The RING domain mutant PIAS3 has a reduced ability to bind Smad3.