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. 2003 Dec 22;101(1):99–104. doi: 10.1073/pnas.0307598100

Fig. 6.

Fig. 6.

Analysis of PIAS3 and PIASy for interactions with p300 and HDAC1. (A) PIAS3 but not PIASy can interact with p300. Myc-PIAS3 or Myc-PIASy along with p300-HA was cotransfected into 293T cells. Cell lysates were immunoprecipitated with the HA antibody and immunoblotted with the Myc antibody. The part of the membrane containing p300-HA was immunoblotted with the HA antibody to detect the immunoprecipitated p300-HA. (B) Both PIAS3 and PIASy can interact with HDAC1. Myc-PIAS3 or Myc-PIASy along with Flag-HDAC1 was cotransfected into 293T cells. Cell lysates were immunoprecipitated with the Flag antibody, followed by immunoblot with the Flag and the Myc antibodies.