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. 2003 Dec 22;101(1):141–146. doi: 10.1073/pnas.2237183100

Fig. 1.

Fig. 1.

LMP1 induces p100 processing to p52 in B lymphocytes and epithelial cells. (A) Lysates of EBV growth transformed lymphoblastoid cell lines, IB4 and 1C12, have high levels of p100 and p52 as detected by SDS/PAGE and immunoblot using p52-specific antibody (Upstate Biotechnology, catalog no. 06-413). (B and C) Lysates of EBV uninfected BJAB B lymphoma or C33A carcinoma cells that stably express LMP1 have higher levels of p52 than BJAB or C33A cells that do not express LMP1 as detected by SDS/PAGE and immunoblot using p52, LMP1 (S12 monoclonal), and tubulin control (Sigma B-5–1-2) antibodies. (D) Lysates of HEK293T cells 24 h after transfection with 1 μg of pSG5-FLMP1 (FLAG tagged) have higher levels of p52 than lysates of cells transfected with vector control plasmid as detected by SDS/PAGE and p52 immunoblot. Asterisks indicate nonspecific bands. The results shown are representative of findings from multiple experiments.