Fig. 2.

LMP1-induced p100 processing is specifically blocked by NIK or IKKα dominant negative mutant alleles but not by the NIK aly dominant negative allele. HEK293T cells were cotransfected with 1 μg of pSG5-FLMP1 and 1 μgof expression plasmid for WT or aly NIK_624–947 (A) or myc-tagged kinase negative IKKα K₄₄A (IKKα KM) (B). After 24 h, cells were harvested, lysed, and analyzed by SDS/PAGE and immunoblot for p52, LMP1, NIK, and myc-tagged IKKα levels. Asterisks indicate nonspecific bands. The results shown are representative of at least two similar experiments for each transfection.